Fruit juice and puree characteristics influence enrichment requirements for real-time PCR detection of Alicyclobacillus acidoterrestris

Table 3 Comparison of detection of A. acidoterrestris in different fruit matrices by real-time PCR and conventional plating using K agar and confirmation of guaiacol formation

Sample	⁰ Brix	Time requirement*	Growth on K Agar		Guaiacol Formation
Sample	⁰ Brix	Time requirement*	24 h	48 h	24 h	48 h
Orange concentrate	43	24 h	-	-	-	-
Apple concentrate	40	24 h	+	+	+	+
Ketchup	27	24 h	-	-	-	-
Tomato paste	26	24 h	+	+	+	+
Apple puree	17	24 h	-	+	-	+
Peach puree	17	24 h	-	+	-	+
Apple juice (filtered)	12	24 h	-	+	-	+
Peach nectar	12	24 h	+	+	+	+
Apple juice (unfiltered)	11	24 h	+	+	+	+
Orange juice (with pulp)	11	24 h	-	+	-	+
Orange juice (no pulp)	10	24 h	-	+	-	+
Tomato sauce	9	24 h	+	+	+	+
Tomato juice	6	24 h	+	+	+	+

ISSN: 2731-9245