Table 3 Chromatographic conditions of the LC-MS/MS system

Column

Waters HSS T3 (1.8 μm)

Injection volume

10 μL

Oven temperature

40 °C

Mobile phase A

Water + 10 mM ammoniumacetate

Mobile phase B

Acetonitrile + 0.1% formic acid

Flow

0.4 mL/min

Gradient

0–0.25 min 2% solvent B

0–7 min linear gradient to 98% solvent B

7–8 min 98% solvent B

8–9 min linear gradient to 2% solvent

9–10 min 2% solvent B

Detector

Triple quadrupole mass spectrometer

Spectrometer

None

Interface

Electrospray ionisation

Potential

5000 V

Temperature

500 °C

Scan type

MRM (Multiple Reaction Monitoring Mode)

Collision gas

Argon