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Table 1 Performance parameters and mass detector settings for the assessed analytes in order of increasing molar mass

From: Chemical assessment of mycotoxin contaminants and veterinary residues in Costa Rican animal feed

Analyte

Molar mass, g/mol

Retention time, min

Target ion, m/z

Fragmentor

Dwell time, ms

Calibration curve equationa or response factor

bLimit of detection, μg kg− 1 (cμg L− 1)

Limit of quantification, μg kg− 1

Mycotoxin contaminants

 Patulin

154.21

24.63

154.3 [M+]

120

71

y = (4.81 × 103 ± 2.69 × 102)x

0.62 (10.1)

1.84

 Citrinin

251.10

23.48

251.0 [M+]

120

56

y = (3.67 × 103 ± 8.11 × 102)x

0.34 (1.9)

1.03

 Deoxynivalenol

296.30

8.69

297.1 [M + H]+ and 319.0 [M + Na]+

120

144

y = (1.61 × 103 ± 4.11 × 102)x

6.31 (105.2)

19.12

 Aflatoxin B1

312.28

21.11

313.0 [M + H]+

140

34

y = (2.62 × 102 ± 3.14 × 101)x

0.40 (6.7)

1.21

 Nivalenol

312.32

5.14

355.1 [M + 2Na]+

140

143

y = (1.73 × 102 ± 2.10 × 101)x

6.42 (107.0)

19.45

 Aflatoxin B2

314.30

20.48

315.0 [M + H]+

140

34

y = (1.01 × 102 ± 3.15 × 101)x

0.14 (2.3)

0.42

 Zearalenone

318.40

24.67

319.0 [M + H]+

120

71

y = (6.75 × 102 ± 3.41 × 101)x

0.26 (4.3)

0.79

 β-zearalenol

320.38

22.16

321.0 [M + H]+

120

56

y = (1.43 × 102 ± 2.55 × 101)x

0.44 (7.3)

1.33

 α-zearalenol

320.38

23.07

321.1 [M + H]+

120

56

y = (4.80 × 103 ± 2.01 × 102)x

0.63 (5.67)

1.91

 Sterigmatocystin

324.30

25.43

327.1 [M + 3H]+

140

95

y = (7.64 × 102 ± 5.54 × 101)x

0.60 (10.0)

1.82

 Aflatoxin G1

328.30

20.48

328.9 [M + H]+

140

34

y = (5.95 × 102 ± 2.33 × 101)x

0.27 (4.5)

0.82

 Aflatoxin M1

328.27

19.13

329.0 [M + H]+

140

34

y = (4.02 × 103 ± 3.61 × 102)x

0.005 (0.8)

0.02

 Aflatoxin G2

330.30

19.87

331.0 [M + H]+

140

34

y = (1.10 × 102 ± 1.12 × 101)x

0.20 (3.3)

0.61

 3-acetoxydeoxynivalenol

338.35

18.01

339.2 [M + H]+

140

71

y = (1.78 × 102 ± 6.65 × 101)x

0.38 (6.3)

1.15

 15-acetoxydeoxynivalenol

338.35

18.26

339.1 [M + H]+

120

71

y = (8.77 × 102 ± 2.15 × 101)x

0.30 (5.0)

0.91

 Fusarenon-X

354.35

15.74

355.0 [M + H]+

140

71

y = (1.40 × 102 ± 1.17 × 101)x

0.29 (4.8)

0.88

 Diacetoxyscirpenol

366.41

16.84

365.0 [C19H25O7]+˙ - H˙

140

71

y = (8.35 × 101 ± 4.31 × 100)x

0.32 (5.3)

0.97

 Ochratoxin B

369.37

22.71

370.0 [M + H]+

120

56

y = (1.23 × 102 ± 1.15 × 101)x

0.21 (3.5)

0.64

 Neosolaniol

382.41

20.79

384.2 [M + 2H]+

140

34

y = (8.74 × 102 ± 9.14 × 101)x

0.61 (10.2)

1.85

 Ochratoxin A

405.40

24.35

404.0 [M + H]+

120

71

y = (1.19 × 102 ± 5.09 × 101)x

0.16 (2.7)

0.48

 HT-2 toxin

424.48

21.38

425.1 [M + H]+

140

56

y = (1.06 × 103 ± 2.19 × 102)x

0.32 (5.3)

0.97

 T-2 toxin

466.47

22.66

484.3 [M + NH4]+

140

71

y = (8.78 × 102 ± 4.54 × 101)x

0.96 (16.0)

2.91

 Enniatin B

639.83

29.94

640.3 [M + H]+

120

95

y = (3.50 × 102 ± 1.07 × 101)x

1.16 (19.2)

3.50

 Enniatin B1

653.86

30.98

654.3 [M + H]+

120

95

y = (2.00 × 103 ± 2.01 × 102)x

0.11 (1.8)

0.34

 Fumonisin B1

721.84

18.88

723.2 [M + 2H]+

100

34

y = (1.19 × 103 ± 1.27 × 102)x

0.08 (1.3)

0.24

 Fumonisin B2

705.84

20.09

706.2 [M + H]+

100

34

y = (3.20 × 102 ± 3.23 × 101)x

0.43 (7.17)

1.30

Veterinary residues

 Sulfamethazine

278.33

9.18

279.0 [M + H]+

120

146

2.61 × 104 ± 6.91 × 103

45.45 (50)

151.52

 Spectinomycin

332.35

1.18

333.1 [M + H]+

140

97

7.53 × 102 ± 1.91 × 101

68.18 (75)

227.27

 Florfenicol

358.20

13.15

339.9 [C12H14Cl2NO4S]+˙ - F˙

120

97

1.33 × 102 ± 2.32 × 101

45.45 (50)

151.52

 Cloramphenicol

323.13

14.51

344.9 [M + Na]+

120

97

3.02 × 102 ± 9.11 × 101

46.36 (51)

154.55

 Penicillin G

334.39

17.28

354.0 [M + Na]+ and 367.0 [M + 2H2O]+

60

83

1.75 × 103 ± 2.67 × 102

97.27 (107)

324.24

 Lincomycin

406.54

7.23

407.1 [M + H]+

120

146

4.32 × 103 ± 1.41 × 102

81.82 (90)

272.73

 4-epianhydrotetracycline

426.42

14.77

427.0 [M + H]+

120

97

1.27 × 103 ± 5.44 × 102

100.01 (105)

303.03

 Methacycline

442.42

13.68

443.0 [M + H]+

120

97

2.33 × 102 ± 7.83 × 101

57.27 (63)

190.91

 Tetracycline

444.44

10.12

445.0 [M + H]+

120

97

2.00 × 103 ± 4.23 × 102

41.82 (46)

139.39

 Doxycycline

444.44

14.08

446.0 [M + 2H]+

120

97

1.17 × 102 ± 5.67 × 101

83.64 (92)

278.79

 4-epitetracycline

444.44

10.23

447.1 [M + 3H]+

140

97

3.19 × 102 ± 3.23 × 101

90.91 (100)

275.48

 Minocycline

457.48

7.14

458.1 [M + H]+

120

146

1.25 × 103 ± 1.01 × 102

71.21 (83)

254.38

 Demeclocycline

464.85

11.43

465.0 [M + H]+

120

97

1.12 × 103 ± 7.34 × 102

41.82 (46)

139.39

 Oxytetracycline

460.44

9.46

461.0 [M + H]+

140

97

2.27 × 102 ± 6.51 × 101

82.73 (91)

275.76

 4-epioxytetracycline

460.44

9.42

462.3 [M + 2H]+

120

146

2.94 × 102 ± 2.21 × 101

101.02 (113)

368.63

 Chlortetracycline

478.88

13.10

479.0 [M + H]+

120

97

1.25 × 103 ± 5.55 × 102

92.12 (102)

301.06

 4-epichlortetracycline

478.88

11.57

479.1 [M + H]+

120

97

6.51 × 102 ± 3.48 × 101

132.73 (146)

442.42

 Virginimycin M1

525.60

21.94

526.1 [M + H]+

120

83

4.46 × 103 ± 2.46 × 102

59.09 (65)

196.97

 Monensin A

670.88

24.56

693.2 [M + Na]+

180

83

1.13 × 104 ± 1.01 × 103

40.91 (45)

136.36

 Erythromycin

733.94

16.83

734.3 [M + H]+

140

83

1.16 × 104 ± 1.76 × 103

80.93 (89)

264.56

 Narasin

765.04

25.20

787.3 [M + Na]+

260

83

1.26 × 103 ± 2.26 × 102

24.55 (27)

81.82

 Virginimycin S1

823.90

23.35

824.1 [M + H]+

120

83

3.47 × 103 ± 3.44 × 102

59.09 (65)

196.97

 Tylosin

916.11

18.05

916.2 [M+]

140

83

5.37 × 103 ± 2.88 × 102

45.45 (48)

151.52

  1. aAll trend line intercepts adjusted to zero as their values are deemed negligible with respect to the slopes and areas. The equations shown are the result of an average of three curves measured independently and under reproducibility conditions
  2. bLimit of detection and quantification calculated as S/N ratio times 3.3 and 10, respectively. Values corroborated by extinction assays
  3. cSmallest concentration detected for each analyte within the calibration curve (experimental data)